Doubled Haploid(DH) Technology

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  • A doubled haploid (DH) is a genotype formed when haploid cells undergo chromosome doubling. Artificial production of doubled haploids is important in plant breeding.

  • For doubled haploids to become a valuable breeding technique, it is necessary that success of doubled haploid production is not genotype dependent, the protocol used is efficient in time and cost.

  • Benefits of this technology are ,Double Haploid technology creates true-breeding lines in a single step, Combined DH & molecular marker technologies result in increased rates of genetic gain, Facilitates multiple trait stacking/pyramiding, Increases efficiency & probability of successful product development , Reduces time to market.

  • Double haploids are a product of modern technology which uses microspores to achieve a uniform parent line (homozygous) from very early generations of breeding material. This technique eliminates the need for several generations of breeding crosses.

  • Double haploids are also used to efficiently develop molecular mapping populations and ensure traits are uniformly expressed in a plant. This one step process replaces previous methods which could take up to six generations of plant growth.

  • Doubled-haploid technology enhances “forward breeding” by allowing hybrids to be bred with new traits (GMO and non-GMO) without locking up the germplasm, and by developing stacks without negative side effects.

  • The doubled-haploid method is much faster and can produce a new, genetically stable inbred line in one year.

  • The doubled-haploid method greatly reduces the time required to develop new parent inbreds.

  • The relevance of DHs to plant breeding has increased markedly in recent years owing to the development of protocols for 25 species. Doubled haploidy already plays an important role in hybrid cultivar production of vegetables, and the potential for ornamental production is being vigorously examined.

  • The other advantages include development of large number of homozygous lines, efficient genetic analysis and development of markers for useful traits in much less time.

  • Tobacco, rapeseed, and barley are the most responsive species for doubled haploid production.

  • Doubled haploid methodologies have now been applied to over 250 species.

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