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- Fibroin is a type of protein created
by Bombyx mori (silkworms) in the production of silk.
- It is a promising scaffold material
for ligament because it is a strong biocompatible protein-based
polymer that exhibits slow degradation rates.
- Its films composed of silk I structure
were first prepared by slow drying process.
- Raw materials of fibroin is silk.
- It can be spun at rates as fast as 10
ml/hr using this electrospinning method as well as it will be
extracted from the cocoons of Bombyx mori silkworms.
- The domesticated silkworm Bombyx mori
silk fibroin consist of two proteins they are a light chain
and heavy chain which are present in a 1:1 ratio and linked by a single disulfide bond.
- As a fibrous protein, silk fibroin
(SF) fibres from Bombyx mori have been used as a biomedical suture material as a long time. because of
their outstanding mechanical properties, in contrast with the catalytic and
recognition functions of globular proteins.
- The degummed silk fibroin was dried at
40 C and atmospheric pressure. Silk fibroin micro- and nanoparticles
were obtained in water-miscible organic solvents.
- Silk fibroin film was prepared
by casting 2 wt.% SF solution in flat-bottomed polystyrene dishes at
room temperature in a flow cabinet.
- Silk fibroin is an
amphiphilic block co-polymer with the molecular mass of the dominant
high molecular weight protein of 390 KDa.
- The variability
demonstrates the sensitivity of silk fibroin to sample manipulation
&necessitated measurements on a large number of independently
prepared samples in order to extract the general features of silk
- It has mainly been applied to wound
healing, successfully performing as man-made blood-vessels ,
surgical sutures and repair materials.
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