Silk Fibroin

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  • Fibroin is a type of protein created by Bombyx mori (silkworms) in the production of silk.
  • It is a promising scaffold material for ligament because it is a strong biocompatible protein-based polymer that exhibits slow degradation rates.
  • Its films composed of silk I structure were first prepared by slow drying process.
  • Raw materials of fibroin is silk.
  • It can be spun at rates as fast as 10 ml/hr using this electrospinning method as well as it will be extracted from the cocoons of Bombyx mori silkworms.
  • The domesticated silkworm Bombyx mori silk fibroin consist of two proteins they are a light chain  and heavy chain which are present in a 1:1 ratio and linked by a single disulfide bond.
  • As a fibrous protein, silk fibroin (SF) fibres from Bombyx mori have been used as a biomedical suture material as a long time. because of their outstanding mechanical properties, in contrast with the catalytic and recognition functions of globular proteins.
  • The degummed silk fibroin was dried at 40 C and atmospheric pressure. Silk fibroin micro- and nanoparticles were obtained in water-miscible organic solvents.
  • Silk fibroin  film was prepared by casting 2 wt.% SF solution in flat-bottomed polystyrene dishes at room temperature in a flow cabinet.
  • Silk fibroin is an amphiphilic block co-polymer with the molecular mass of the dominant high molecular weight protein of 390 KDa.
  • The variability demonstrates the sensitivity of silk fibroin to sample manipulation &necessitated measurements on a large number of independently prepared samples in order to extract the general features of silk fibroin gelation.
  • It has mainly been applied to wound healing, successfully performing as man-made blood-vessels , surgical sutures and repair materials.

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