facilitate trade whilst preventing the spread of disease, it is
essential to have rapid, simple and accurate methods by which to
detect and identify plant pathogens. In the European Union, plant
passports are issued to guarantee plant health, but must be validated
to be worthwhile. Tests and test protocols vary between countries, and
in order to harmonise testing and build confidence, there is a need to
devise standardised tests and methodologies that can be used to detect
and identify economically important pathogens in different countries.
These tests must be specific, robust and simple.
detection by means of rapid and sensitive tests would assist control
measures. They would also help to minimize chemical inputs and thereby
be an important feature of attempts to develop sustainable crop
AED-2000L instrument combined with the SP-1L probe set and accessories
has been designed to handle a number of insect pest detection
- The new detection
method -- designed using a DNA-amplification technique called
polymerase chain reaction -- consistently detected even minute traces
of the disease-causing strain of F. solani in soil and in plant
tissues grown in both the laboratory and in fields across Illinois.
Its presence was found even from samples that were thought to be free
of disease. The PCR-based method uses two primers that were designed
from the extraction and genetic alteration of material taken from
various strains of F. solani.
- Modern scientific
methods can be used for mass culture and formulations, understanding
the chemistry of nematicidal products and molecules etc. There is a
need for more basic research in development of techniques for
detection, identification and management of nematodes.
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