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Information @ a Glance

Introduction

  • Recombinant DNA is DNA that has been created artificially. DNA from two or more sources is incorporated into a single recombinant molecule.
  • Recombinant DNA is a form of artificial DNA that is engineered through the combination or insertion of one or more DNA strands, thereby combining DNA sequences that would not normally occur together.

  • Recombinant DNA works when the host cell expresses protein from the recombinant genes. 

  • Recombinant DNA is used widely today to create large amounts of protein for treating certain illnesses.

Technology

  • The technique used for recombinant DNA was first discovered by Stanley Cohen and Herbert Boyer in 1973, specifically in their November 1973 publication of “Construction of Biologically Functional Bacterial Plasmids in vitro”.

  • Recombinant technology begins with the isolation of a gene of interest.

  • Recombinant DNA is the technology that allows us to insert genes from one organism into another to make it produce a protein product, copy the gene multiple times, or give it a new trait.

Production

  • The first step in making recombinant DNA is to isolate donor and vector DNA.

  • There are three different methods by which Recombinant DNA is made. They are Transformation, Phage Introduction, and Non-Bacterial Transformation.

  • Production of recombinant proteins in eukaryotic systems generally takes place in  yeast and filamentous fungi.

  • The basic procedure is to extract and cut up DNA from a donor genome into fragments containing from one to several genes and allow these fragments to insert themselves individually into opened-up small autonomously replicating DNA molecules such as bacterial plasmids

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