Recombinant DNA is DNA that has been created artificially. DNA
from two or more sources is incorporated into a single
Recombinant DNA is a form of artificial DNA that is
engineered through the combination or insertion of one or more
DNA strands, thereby combining DNA sequences that would not
normally occur together.
Recombinant DNA works when the host cell expresses protein from
the recombinant genes.
is used widely today to create large amounts of protein for
treating certain illnesses.
used for recombinant DNA was first discovered by Stanley Cohen
and Herbert Boyer in 1973, specifically in their November 1973
publication of “Construction of Biologically Functional
Bacterial Plasmids in vitro”.
Recombinant technology begins with the isolation of a gene of
is the technology that allows us to insert genes from one
organism into another to make it produce a protein product, copy
the gene multiple times, or give it a new trait.
The first step in
making recombinant DNA is to isolate donor and vector DNA.
There are three different methods by which Recombinant DNA is
made. They are Transformation, Phage Introduction, and
Production of recombinant proteins in eukaryotic systems
generally takes place in yeast and filamentous fungi.
procedure is to extract and cut up DNA from a donor genome into
fragments containing from one to several genes and allow these
fragments to insert themselves individually into opened-up small
autonomously replicating DNA molecules such as bacterial
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